Figure 4

Growth and polyhydroxybutyrate (PHB) production by recombinant and wild-type H. campaniensis LS21 fed with mixed substrates over a period of 65 days under unsterile and continuous conditions. (A) Productions of cell dry weights (CDW) and PHB by wild-type and recombinant H. campaniensis LS21 grown in mixed substrates over a period of 65 days under unsterile and continuous conditions. (B) Both wild-type and recombinant H. campaniensis LS21 maintained a relatively stable pH during different phases of growth without automatic pH adjustments. (C) Results of PCR assays for wild-type and recombinant H. campaniensis LS21 cultures from their respective unsterilized fermentations on day 65. The primers were designed based on the conserved sequences of H. campaniensis LS21. Eleven colonies were randomly selected from Petri disks on day-65 cultures, respectively, for the PCR assays. M: marker, lanes 1 to 11: culture of recombinant H. campaniensis LS21 as the PCR template; lanes 12 to 22: culture of wild-type H. campaniensis LS21 as the PCR template.