Figure 6
From: Evolution of substrate specificity in bacterial AA10 lytic polysaccharide monooxygenases
Evolution of chitinolytic and cellulolytic AA10 genes. (a) Plot of pairwise estimated dN and dS for genes from cellulolytic and chitinolytic clades, clade A (circles) and clade D (diamonds), respectively. Values were calculated by pairwise comparison of all genes within each clade and filtered to remove insignificant values (see text). Also shown are trend lines for dN/dS ratios of 1 and 0.2, representing approximate thresholds for positive and negative selection. Site-specific estimation of dN/dS ratios for clade A (b) and clade D (c). Positively selected residues are colored in red, neutral in grey, and negative in blue (x-axis corresponds to protein sequence and y-axis to the posterior probability of the estimation). dN/dS ratios were also mapped onto the CBP21 structure 2BEM, and the modeled structure for SACTE_3159. Colors correspond to selection rates described above.