Fourier transform infrared spectroscopy as a method to study lipid accumulation in oleaginous yeasts

Table 4 Mean squared distances (MSD) (1,500 and 1,350 cm ^-1 ) S. cerevisiae versus R. toruloides

Fatty acid standard	S.c.0 h	S.c.72 h	R.t.0 h	R.t.72 h	Δ% S.c.	Δ% R.t.	Δ% (R.t.- S.c.)
Linolenic	292.12	151.72	299.24	84.48	48.06	71.77	23.71
Linoleic	341.28	202.45	332.15	121.66	40.68	63.37	22.69
Oleic	362.75	221.33	349.84	135.73	38.99	61.2	22.21
Palmitic	326.31	284.29	322.42	233.57	12.88	27.56	14.68
Palmitoleic	351.93	224.39	334.66	140.91	36.24	57.89	21.65
Stearic	265.9	229.43	311.99	216.98	13.71	30.45	16.74

MSD among the centroids in the three-dimensional PCA projection. Mean squared distances (MSD) among the centroids of the sets in the three-dimensional principal components analysis (PCA) projection have been reported to evaluate the distance between the producer and the control strain with the lipid standards for the range 1,500 and 1,350 cm^-1. In addition, the percentage of the difference between the MSDs has been reported (see Methods for details). Moreover, the last column represents the difference between Δ% R. toruloides and Δ% S. cerevisiae; S.c., S. cerevisiae; R.t., R. toruloides.

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