Time course of glucose and cellobiose evolution during biochemical conversion of cellulose. (A) Enzymatic hydrolysis by cellulase in the presence of cellobiase. (B) Enzymatic hydrolysis by cellulase in the presence of heat-inactivated cellobiase. (C) Conversion by C. curvatus cells according to the SSLP process with heat-inactivated cellobiase. Initial cellulose concentration was 40 g/L. Cellulase and cellobiase were loaded at 15 FPU and 30 CBU per gram of cellulose. Hydrolysis was done at 50°C, 200 rpm in 0.05 M citrate buffer (pH 4.8). C. curvatus cells were cultured at 30°C, 200 rpm. CBU, cellobiase unit; FPU, filter paper unit; SSLP, simultaneous saccharification and lipid production.