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Figure 3 | Biotechnology for Biofuels

Figure 3

From: Deletion of meso-2,3-butanediol dehydrogenase gene bud C for enhanced D-2,3-butanediol production in Bacillus licheniformis

Figure 3

PCR verification of recombinant strains. (A) PCR verification of bud C knock-out strain. M, DL5000 marker (5,000, 3,000, 2,000, 1,500, 1,000, 750, 500, 250 and 100 bp, up to bottom); lane 1, negative control (PCR product from the genomic DNA of B. licheniformis WX-02); lanes 2 and 3, fragment amplified from the genomic DNA of B. licheniformis WX-02ΔbudC. (B) PCR verification of complementary strain of B. licheniformis WX-02 Δbud C/pHYbud C. M, DL5000 marker; lane 1, PCR product of fusion fragment of P43-bud C-TamyL from recombinant strain B. licheniformis WX-02 ΔbudC/pHYbudC.

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