Cellulases without carbohydrate-binding modules in high consistency ethanol production process

Table 1 Enzyme mixtures and their loading in the small scale experiments

Enzymes	Components in enzyme mixtures		Dosage of enzymes molar basis: μmol/g DM		Dosage of enzymes weight basis: mg/g DM
Enzymes	CBM+	CBM-	Low	High	Low	High
Ta Cel7A		+	0.20	0.40	9.4	18.8
Ta Cel7A + Tr CBM	+		0.20	0.40	10.6	21.2
Ta Cel5A		+	0.05	0.10	1.7	3.4
Ta Cel5A + Ct CBM	+		0.05	0.10	2.1	4.2
Ta Xyn10A	+	+	0.05	0.10	1.6	3.3
Ta Cel3A	+	+	0.01	0.02	1,000.0^a	2,000.0^a

Low and high dosages of enzyme proteins (as μmol or mg/g DM substrate) used in laboratory-scale hydrolysis experiments. Enzyme mixtures showing the components in CBM+ and CBM- enzyme mixtures. The total protein loading of the used enzymes were on the low level, 14.3 mg/g (CBM+) and 12.7 mg/g (CBM-), and on the high enzyme level, 28.7 mg/g (CBM+) and 25.5 mg/g (CBM-). ^aBG dosed as nkat/g DM. BG, β-glucosidase; CBM, carbohydrate-binding module; DM, dry matter.

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