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Table 2 Sugar yields in small-scale hydrolysis experiments of pretreated wheat straw using various protein loadings

From: Cellulases without carbohydrate-binding modules in high consistency ethanol production process

Total loaded protein (mg/g DM) Hydrolysis yield, % of total carbohydrates
CBM-containing CBM-lacking
7.2 39.4 (6.5) 37.9 (3.6)
14.4 60.5 (5.4) 60.0 (0.8)
17.3 84.1 (1.5) 78.5 (0.4)
28.8 93.3 (0.5) 81.9 (1.3)
28.8a 94.4 (1.6) 90.7 (1.9)
43.2 88.6 (4.6) 86.6 (2.2)
  1. Sugar yields in small-scale hydrolysis experiments of pretreated wheat straw (consistency 25%) using various protein loadings by enzyme mixtures containing CBH I with (CBM+) or without (CBM-), EG II, XYL and BG of Thermoascus aurantiacus. The ratio of enzymes (protein-based) was CBH I:EG II:XYL 6:1:1. BG was dosed as nkat/g dw following the ratios of other proteins. aAddition of PEG, 1% of substrate DM. Hydrolysis 72 hours, temperature 50°C. Hydrolysis yields are expressed as the percentage of total carbohydrates, measured as reducing sugars. Standard deviations are in parentheses. BG, β-glucosidase; CBH, cellobiohydrolase; CBM, carbohydrate-binding module; DM, dry matter; EG, endoglucanase; PEG, polyethylene glycol; XYL, xylanase.