Table 1 Ethylene productivities in EFE-expressing microbes
Host | Native, vector, or integrated EFE expression | Promoter | Temperature, °C | Feedstock | Rate of ethylene production. (μmol/gCDW/h) | Ref. |
|---|---|---|---|---|---|---|
Pseudomonas syringae (Kudzu) | Native | Kudzu | 30 | LB + 0.5% glucose | 39.0 | 18 |
P. syringae (Kudzu) | Native + vector (RS1010) | Kudzu, npt | 30 | LB + 0.5% glucose | 312.0 | 18 |
Escherichia coli (JM109) | Vector (pUC19) | Kudzu | 37 | Modified LB | 10.9 | 15 |
E. coli (DH5α) | Vector (pUC18) | lac | 25 | LB | 625.0 | 16 |
E. coli (JM109) | Vector (pBR322) | tac | 25 | LB | 412.9 | 16 |
E. coli (JM109) | Vector (RS1010) | npt | 30 | LB + 0.5% glucose | 55.2 | 18 |
E. coli (DH5α) | Vector (RS1010) | lac/trc | 30 | LB | 22.8 | 26 |
E. coli (MG155) | Vector (pBR322) | psbA | 30 | M9 + 1% glucose | 30.0 | UP |
Saccharomyces cerevisiae (batch) | Vector (pYX212) | tpi | 30 | YNB + 1% glucose + glutamate | 21.4 | 17 |
S. cerevisiae (chemostat) | Vector (pYX212) | tpi | 30 | CBS + 1% glucose + (NH4)2SO4 | 1083.8 | 42 |
S. cerevisiae (chemostat) | Vector (pYX212) | tpi | 30 | CBS + 1% glucose + glutamate | 1151.5 | 41 |
S. cerevisiae (chemostat) | Vector (pYX212) | tpi | 30 | CBS + 1% glucose + glutamate + arginine | 492.0 | 42 |
Synechococcus 7942 | Vector (pUC303) | psbA1 | 25 | BG11 | 84.8 | 23 |
Synechococcus 7942 | Integrated (psbAI) | psbA1 | 28 | BG11 | 80.5 | 24 |
Synechocystis 6803 | Vector (RS1010) | lac/trc | 30 | BG11 | 26.0 | 26 |
Synechocystis 6803 | Integrated (slr0168) | psbA | 30 | BG11 | 111.6 | UP |
Trichoderma viride | Integrated (random) | cbhI | 30 | MM + 2% cellulose +0.2% peptone | 0.093 | 19 |
Trichoderma reesei | Integrated (random) | pgk | 30 | MM + 2% wheat straw | 0.716 | 20 |
Pseudomonas putida | Vector (RS1010) | npt | 30 | LB + 0.5% glucose | 1050.0 | 18 |
P. putida | Integrated (five 16S rDNA sites) + vector (pBBR1MCS2) | rrn | 28 | LB | 2859.2 | 48 |