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Table 1 Ethylene productivities in EFE-expressing microbes

From: Ethylene-forming enzyme and bioethylene production

Host Native, vector, or integrated EFE expression Promoter Temperature, °C Feedstock Rate of ethylene production. (μmol/gCDW/h) Ref.
Pseudomonas syringae (Kudzu) Native Kudzu 30 LB + 0.5% glucose 39.0 18
P. syringae (Kudzu) Native + vector (RS1010) Kudzu, npt 30 LB + 0.5% glucose 312.0 18
Escherichia coli (JM109) Vector (pUC19) Kudzu 37 Modified LB 10.9 15
E. coli (DH5α) Vector (pUC18) lac 25 LB 625.0 16
E. coli (JM109) Vector (pBR322) tac 25 LB 412.9 16
E. coli (JM109) Vector (RS1010) npt 30 LB + 0.5% glucose 55.2 18
E. coli (DH5α) Vector (RS1010) lac/trc 30 LB 22.8 26
E. coli (MG155) Vector (pBR322) psbA 30 M9 + 1% glucose 30.0 UP
Saccharomyces cerevisiae (batch) Vector (pYX212) tpi 30 YNB + 1% glucose + glutamate 21.4 17
S. cerevisiae (chemostat) Vector (pYX212) tpi 30 CBS + 1% glucose + (NH4)2SO4 1083.8 42
S. cerevisiae (chemostat) Vector (pYX212) tpi 30 CBS + 1% glucose + glutamate 1151.5 41
S. cerevisiae (chemostat) Vector (pYX212) tpi 30 CBS + 1% glucose + glutamate + arginine 492.0 42
Synechococcus 7942 Vector (pUC303) psbA1 25 BG11 84.8 23
Synechococcus 7942 Integrated (psbAI) psbA1 28 BG11 80.5 24
Synechocystis 6803 Vector (RS1010) lac/trc 30 BG11 26.0 26
Synechocystis 6803 Integrated (slr0168) psbA 30 BG11 111.6 UP
Trichoderma viride Integrated (random) cbhI 30 MM + 2% cellulose +0.2% peptone 0.093 19
Trichoderma reesei Integrated (random) pgk 30 MM + 2% wheat straw 0.716 20
Pseudomonas putida Vector (RS1010) npt 30 LB + 0.5% glucose 1050.0 18
P. putida Integrated (five 16S rDNA sites) + vector (pBBR1MCS2) rrn 28 LB 2859.2 48
  1. For comparison, reported rates have been converted to μmol/gCDW/h.
  2. CDW, cell dry weight; EFE, ethylene-forming enzyme.