Cloning of JcSDP1 gene from Jatropha plants, and complementation of the Arabidopsis sdp1-5 mutant. (A) Deduced amino acid sequence of JcSDP1 and alignment to the AtSDP1 sequence. Bold line, patatin domain; star (*****); site-1 protease (S1P) target sequence RXXL; GXGXXG, oxyanion hole motif; GXSXG, lipase consensus motif with catalytic serine. (B) Complementation of sdp1-5 with JcSDP1. WT (Col-0), sdp1-5, and 35S:JcSDP1/sdp1-5 transgenic plants were grown on MS medium with or without sucrose (1%).