Comparison of three pretreatments on subsequent enzyme hydrolysis of four species of forbs. A. Dilute acid. B. AFEX. C. AHP. C/HTec2 refers to a 3:1 mixture of Cellic™ CTec2 and HTec2. Acc1000 is Accellerase 1000. Numbers after the enzyme names indicate the loadings in mg protein/gm glucan. Biomass loading was 2 mg glucan/ml, incubation temperature was 50°C, and incubation time was 48 hours. AFEX, ammonia fiber expansion; AHP, alkaline hydrogen peroxide; Glc, total glucose.