Figure 5

Microscopic images of SYTOX green-stained Synechocystis . Synechocystis/pKT230 grown under continuous red light (A and B) or under red and green light (C and D), and Synechocystis/pKTLYS grown under red light (E and F) or under red and green light (G and H) were observed by visible-light microscopy (A, C, E, and G) and by fluorescence microscopy (B, D, F, and H). All cells were grown for 64 hours and then incubated with 5 μM SYTOX Green nucleic acid stain for five minutes at room temperature. Fluorescence microscopy was performed on a Biozero BZ- 8000 (Keyence, Osaka, Japan) (excitation: 485 nm, emission: 520 nm).