Figure 2
From: Production of free monounsaturated fatty acids by metabolically engineered Escherichia coli
SDS-PAGE analysis of different recombinant proteins expressed in E. coli . Recombinant protein expression was induced using 0.1 mM isopropyl-ß-D-thiogalactopyranoside (IPTG) for a cultivation time of 4 h at 37°C. Lane M, prestained protein molecular weight marker; lane 1, control strain harboring pET30a; lane 2, crude cells extracts from strain BL21/pA-AtFatA; lane 3, crude cells extracts from strain BL21/pE-ssi2; lane 4, crude cells extracts from strain BL21/pE-AtFatAssi2; lane 5, crude cells extracts from strain BL21/pA-acc; lane 6, crude cells extracts from recombinant strain harboring both pE-AtFatA and pA-acc. The bands corresponding to the individual proteins were indicated by an arrow.