Figure 5
From: Production of free monounsaturated fatty acids by metabolically engineered Escherichia coli
Comparison of free fatty-acid production and the yields on glucose of several different strains. BL21/pA-AtFatA, strain BL21 star(DE3) expressing Arabidopsis thioesterase; BL21/pE-AtFatAssi2, strain BL21 star(DE3) expressing Arabidopsis thioesterase and fatty-acid desaturase; BL21ΔfadD/pE-AtFatAssi2, knockout of native fadD while expressing AtFatA and SSI2; BL21ΔfadD/pE-AtFatAssi2&pA-acc, further overexpression native of E. coli acetyl-CoA carboxylase. Data were obtained after each strain was induced for 16 h in liquid M9 minimal medium supplemented with 1 mM MgSO4 and 20 g/L glucose except for the recombinant strain BL21ΔfadD/pE-AtFatAssi2&pA-acc (12 h). FFA, free fatty acid.