Figure 5

Schematic overview of the n-butanol degradation pathway in A. adeninivorans . Arxula is able to use n-butanol as the sole carbon and energy source, by converting it into the central metabolite acetyl-CoA by ß-oxidation, to finally generate succinate in the peroxisomes. A genome-mining approach led to the proposal of the pathway shown here. The figure shows the reversible (double headed arrow) and irreversible (single headed arrow) reactions catalyzed by the corresponding enzymes (rectangular square) and the cofactors (ATP/AMP, NAD+/NADH) necessary for n-butanol degradation. Black marked symbols indicate intermediates occurring several times in the pathway. AMP, Adenosine monophosphate; ATP, Adenosine triphosphate; CoA, coenzyme A; NAD, Nicotinamide adenine dinucleotide; PP, phosphate.