Starch screening and content determination. (A) Iodine vapour staining on starchless mutants. C. reinhardtii strain BAFJ5 was included as a control. The staining was performed after 9 days of nitrogen deprivation (TAP-N plates) to enhance the visibility of the iodine staining which is normally interfered by the chlorophyll. Starch content (% of DW) for (B) nitrogen-replete and (C) nitrogen-depleted conditions over time. The values are derived from biological duplicate cultures. Deviation from the duplicate average is indicated by the error bars. DW, dry weight; slm, starchless mutant; TAP, Tris-Acetate-Phosphate; wt, wild type.