Figure 3
Expression and activity of LDH and PK in mutant strains of Synechocystis . (A) Coomassie Brilliant Blue (CBB)-stained gel of cell-free extracts of Synechocystis mutant strains showing overexpression of the LDH of L. lactis (*), the PK of E. faecalis (#), and the PK of E. coli (>). The vertical line indicates a composite gel. Lanes are aligned according to the pattern of the wild-type proteins visible in the cell-free extract. (B) Enzymatic activity of LDH in cell-free extracts of relevant mutant strains, showing the relative increase with respect to the benchmark strain SAA023. (C) Enzymatic activity of PK in cell-free extracts with (+) and without (-) fructose-1,6-bisphosphate (FBP), showing the activity relative to the reference strain SAW001. Error bars represent the SD of biological replicates (n = 3); if error bars are not visible, they are smaller than the symbol of the data point; ain (B) and (C) the values represent the mean of biological duplicates, and bin (C) the mean of technical triplicates. In both (B) and (C) the black bar represents the mean, and the open triangles represent the biological, or technical replicates, respectively.