Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803

Table 3 Analysis of the flux control coefficient of LDH in recombinant Synechocystis strains

Strain	Relative activity [E]	Relative flux [J]	Flux control coefficient [C_J^E]
Wild type	0	0	n.a.
SAA024*	0	0	n.a.
SAA005*	0.09 ± 0.04	0.20 ± 0.11	0.99
SAA025*	0.91 ± 0.05	0.78 ± 0.06	0.92
SAW001	0.75 ± 0.01	1.09 ± 0.13	0.93
SAA023	1.00 ± 0.06	1.00 ± 0.10	0.91
SAA026	1.78 ± 0.55	1.87 ± 0.08	0.84
SAW035	5.25 ± 1.03	4.69 ± 0.37	0.59
SAW039	7.59 ± 1.51	5.21 ± 0.70	0.50
SYW003	0.68 ± 0.30	0.80 ± 0.07	n.a.
SYW004	0.71 ± 0.01	1.05 ± 0.23	n.a.
SAW041	8.15 ± 0.53	26.67 ± 2.16	n.a.

Enzyme activity and lactate production rate are expressed relative to the benchmark strain SAA023. Flux control coefficient values are determined from the slope of the tangent to the fitted curve in Figure 4, for the respective relative activity values. Data for SAA005, SAA024, and SAA025 (*) were taken from [14]. Error ranges represent the SD of biological replicates (n = 3), except for SAW001, SYW003, and SYW004 where n = 2; n.a. not applicable.

ISSN: 2731-3654