Figure 2

Growth curve (cell count) for CC124 (a) and the sta6-1 (b) strains, in nitrogen-depletion (diamond) and -repletion (square) TAP medium. Each data point represents three replicates. Arrows indicate the inoculation points of the 25,000 cells from the top 2% of cells sorted using flow cytometry (Day 0 indicates inoculation of 25,000 cells from the top 2% of 0 hour seed culture). Abbreviations: TAP, Tris Acetate Phosphate liquid Medium.