Figure 3

Flow cytograms of adaptive evolved strains of CC124 (a) and sta6-1 (b) in nitrogen-depletion TAP medium. (a) CC124 - Blue and purple representing unstained cells of day 19 and 77, respectively. Red and green representing BODIPY 505/515-stained cells of day 19 and 77, respectively. (b) sta6-1 - Blue and purple representing unstained cells of day 14 and 43, respectively. Red and green representing BODIPY 505/515-stained cells of day 14 and 43, respectively. The mean values of histograms of unstained and BODIPY 505/515-stained cells of CC124 of day 19 and 77 were 6.7, 10.98, 1724.45, and 2206.01, respectively. The mean values of histograms of unstained and BODIPY 505/515-stained cells of sta6-1 of day 14 and 43 were 5.2, 9.54, 1138.34, and 2427.78, respectively. Abbreviations: TAP, Tris Acetate Phosphate liquid Medium; BODIPY, 4,4-difluoro-1,3,5,7-tetramethyl-r-bora-3a,41-diaza-s-indacene.