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Table 3 ANOVA for hydrogen production by T. aotearoense SCUT27/ Δldh with SCB hydrolysates as substratea

From: Optimization of key factors affecting hydrogen production from sugarcane bagasse by a thermophilic anaerobic pure culture

Factors

Sum of squares

Degrees of freedom

Mean square

F-value

P-value

 

Model

18368.05

5

3673.61

13.97

0.0016

significant

X 1

740.37

1

740.37

2.82

0.1373

 

X 2

4796.85

1

4796.85

18.24

0.0037

 

X 1 X 2

150.06

1

150.06

0.57

0.4747

 

X 1 2

4654.76

1

4654.76

17.70

0.0040

 

X 2 2

3230.99

1

3230.99

12.29

0.0099

 

Residual

1840.87

7

262.98

   

Lack of fit

1656.83

3

552.28

12.00

0.0181

significant

Pure error

184.04

4

46.01

   

Cor total

20208.92

12

    
  1. aCoefficient of determination (R2) = 0.9089. A model with an F-value of 13.97 implies that the model is significant. There is only a 0.16% chance that a model F-value this large could occur due to noise. Values of “Prob>F” less than 0.0500 indicate that model terms are significant. In this case B, A2, B2 are significant model terms. The “Lack of fit F-value” of 12.00 implies that the lack of fit is significant. There is only a 1.81% chance that a lack of fit F-value this large could occur due to noise. The “Pred R-Squared” of 0.3684 is not as close to the “Adj R-Squared” of 0.8438 as one might normally expect. This may indicate a large block effect or a possible problem with a model and/or data. Things to consider are model reduction, response transformation, and outliers, among others. “Adeq Precision” measures the signal-to-noise ratio. A ratio greater than 4 is desirable. A ratio of 10.962 indicates an adequate signal. This model can be used to navigate the design space.