Figure 2

Adaptive evolution of the rationally engineered strain expressing the xylose isomerase pathway. The adaptive evolution of the rationally engineered strain (SXA-R2P) was performed in the exponential cell growth phase transfer set with different inoculum sizes of low (0.5%: black), medium (1%: grey), and high (5%: white). The graph shows representative strains with improved cell growth on xylose. The strains adapted in the culture with low inoculum size showed the highest improvement in the cell growth.