Systematic and evolutionary engineering of a xylose isomerase-based pathway in Saccharomyces cerevisiae for efficient conversion yields

Table 1 Saccharomyces cerevisiae strains used in this study

Strains	Characteristics
BY4741 Δgre3	Mat a, his3 Δ1, leu2 Δ0, met15 Δ0, ura3 Δ0, YHR104w::kanMX4
BY4741 Δpho13	Mat a, his3 Δ1, leu2 Δ0, met15 Δ0, ura3 Δ0, YDL236w::kanMX4,
SXA-R1	BY4741 Δgre3, URA::GPDp-xylA3-CYC1t-TEFp- XKS1-CYC1t*
SXA-R2	BY4741 Δgre3, URA::GPDp-xylA3-CYC1t-TEFp- XKS1-CYC1t, Leu:: GPDp-xylA3-RPM1t-TEFp- tal1-CYC1t
SXA-R1P	SXA-R1, YDL236w :: His
SXA-R2P	SXA-R2, YDL236w:: His
SXA-R2P-E	Evolved strain of SXA-R2P

ISSN: 2731-3654