Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP + -linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana

Table 3 The activity of XR and XDH present in crude cell-free extract obtained from disrupted cells of C. sorokiniana ^a

^aThe non-induced and induced algal cells were washed with sterile distilled water and re-suspended in 8 mg DCW per mL in 50 mL minimal medium supplemented with 5 mM KNO₃ and 40 mM D-xylose or D-glucose under light for 24 hours.
^bThe activity of XR was measured at pH 6.0 and 40°C with NADPH as a cofactor.
^cThe activity of XDH was measured at pH 8.0 and 40°C with NADP⁺ as a cofactor.
ND, Not detected.

ISSN: 2731-3654