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Table 3 The activity of XR and XDH present in crude cell-free extract obtained from disrupted cells of C. sorokiniana a

From: Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP + -linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana

  Induced Non-induced
  D-xylose D-glucose D-xylose D-glucose
XRb (U mg-1 protein) 0.021 ND ND ND
XDHc (U mg-1 protein) 0.026 ND ND ND
  1. aThe non-induced and induced algal cells were washed with sterile distilled water and re-suspended in 8 mg DCW per mL in 50 mL minimal medium supplemented with 5 mM KNO3 and 40 mM D-xylose or D-glucose under light for 24 hours.
  2. bThe activity of XR was measured at pH 6.0 and 40°C with NADPH as a cofactor.
  3. cThe activity of XDH was measured at pH 8.0 and 40°C with NADP+ as a cofactor.
  4. ND, Not detected.