Observation and determination of TAG droplets in Chlorella ellipsoidea under nutrient-limited conditions. (A) WT strain; (B) Dof4-1; (C) Dof4-3; (D) Dof4-5; (E) Fluorescence intensity of Chlorella ellipsoidea. The data represent the means ± SD of three replicate experiments and were analyzed by Student’s t-test (n = 8). *P <0.05. TAG, triacylglycerol; WT, wild-type Chlorella ellipsoidea.