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Table 1 Phenotypic sterol analysis of YUG37 parent and YUG37- ERG1 mutant

From: Co-production of ethanol and squalene using a Saccharomyces cerevisiae ERG1 (squalene epoxidase) mutant and agro-industrial feedstock

DOX   Sterol composition (mg sterol g-1) Total Biomass Squalene titre
(μg mL-1) Strain a14α-DM b14α-M Ergosterol Squalene (mg g-1) (g L-1) (mg L-1)
0 YUG37 1.45 [0.27] 0.33 [0.03] 3.27 [0.39] 0.16 [0.08] 5.31 [0.19] 3.75 [0.35] 0.50 [0.11]
  YUG37- ERG1 1.51 [0.29] 0.39 [0.07] 3.08 [029] 0.40 [0.03] 5.28 [0.24] 4.55 [0.21] 1.76 [0.02]
0.025 YUG37 1.30 [0.16] 0.20 [0.06] 3.09 [0.35] 0.17 [0.13] 4.75 [0.02] 4.25 [0.21] 0.55 [0.31]
  YUG37- ERG1 1.55 [0.09] 0.40 [0.03] 2.43 [0.08] 3.57 [0.20] 7.88 [0.31] 4.30 [0.28] 15.04 [1.42]
0.05 YUG37 1.47 [0.22] 0.26 [0.03] 3.03 [0.14] 0.30 [0.04] 4.98 [0.19] 3.90 [0.14] 1.23 [0.03]
  YUG37- ERG1 0.78 [0.10] 0.40 [0.08] 2.20 [0.09] 4.24 [0.30] 7.55 [0.33] 2.50 [0.28] 10.31 [0.79]
0.5 YUG37 1.36 [0.22] 0.24 [0.06] 2.79 [0.32] 0.23 [0.06] 4.77 [0.30] 3.95 [0.07] 1.02 [0.14]
  YUG37- ERG1 0.45 [0.01] 0.17 [0.02] 1.58 [0.12] 6.75 [0.20] 8.96 [0.06] 1.75 [0.21] 11.93 [1.27]
5 YUG37 1.54 [0.29] 0.29 [0.06] 3.06 [0.42] 0.20 [0.10] 5.03 [0.11] 3.60 [0.42] 0.57 [0.12]
  YUG37- ERG1 0.21 [0.04] 0.08 [0.04] 1.49 [0.02] 7.66 [0.01] 9.45 [0.08] 1.48 [0.11] 11.30 [0.82]
50 YUG37 1.21 [0.23] 0.23 [0.01] 2.93 [0.26] 0.14 [0.06] 4.63 [0.27] 3.75 [0.35] 0.59 [0.05]
  YUG37- ERG1 0.11 [0.03] 0.02 [0.01] 1.50 [0.03] 7.85 [0.02] 9.48 [0.10] 1.39 [0.12] 10.87 [0.93]
  1. All cultures grown at 30°C, 180 rpm for 48 h on YPD medium. Mean values (n = 3 [±SD]); DOX = doxycycline. Maximum squalene content and yield are emboldened.
  2. a = sum of all 14α-demethylated sterols; b = sum of 14α-methylated sterols.