Co-production of ethanol and squalene using a Saccharomyces cerevisiae ERG1 (squalene epoxidase) mutant and agro-industrial feedstock

Table 2 Growth parameters for YUG37- ERG1 cultured on GJ at 20°C in the Bioscreen

DOX	ΔOD	Lag phase (h)	T_½Max (h)	DT_min(h)
(μg mL^-1)	ΔOD	Lag phase (h)	T_½Max (h)	DT_min(h)
0	1.69 [0.01]	11.3 [0.4]	21.8 [0.4]	3.8 [0.4]
0.025	1.66 [0.01]	11.4 [0.2]	22.6 [0.5]	4.3 [0.1]
0.05	1.63 [0.01]	11.5 [0.4]	24.1 [1.2]	5.4 [0.2]
0.1	1.54 [0.01]	11.5 [0.7]	28.9 [0.9]	6.0 [0.7]
0.5	1.43 [0.02]	11.8 [0.4]	31.5 [0.7]	8.6 [0.5]
1	1.34 [0.02]	11.5 [0.4]	35.8 [0.4]	10.3 [0.4]
5	1.35 [0.01]	11.5 [0.7]	38.0 [2.8]	10.3 [0.7]
50	1.34 [0.02]	10.9 [0.5]	37.0 [1.4]	10.4 [0.9]

Mean values (n = 3 [±SD]). DOX = doxycycline; ΔOD = Maximum minus minimum optical density reading at 600 nm; Lag phase = Length of time culture remains at <-10% of maximum OD; T_½Max = time taken to achieve half maximal culture growth (maximum OD minus minimum OD × 0.5); DT_min = fastest observed doubling time.

ISSN: 2731-3654