Figure 4

Activity profiling of Laetisaria arvalis CAZymes and their potential to improve the conversion of pretreated wheat straw. A: Clustering of the activities related to lignocellulose degradation in L. arvalis secretomes at day 10. The degree of activity of L. arvalis and Trichoderma reesei CL847 secretomes on the respective substrates is represented by a color scale with different strengths of red. The enzymatic activities of L. arvalis secretomes produced during growth on Avicel (AVI), wheat straw (WS), wheat straw residue following traditional saccharification (WS-R) and maize bran (MB) were determined on a library of substrates, pGlc, para-nitrophenol-β-D-glucose; pLac, para-nitrophenyl-β-D-lactose; pCel, para-nitrophenyl-β-D-cellobiose; pCel3, 2-chloro-4-nitrophenyl-β-D-cellotrioside; CMC, carboxymethyl cellulose; AVI, Avicel; DCPIP, 2,6-dichloro-phenol-indophenol; pXyl, para-nitrophenyl-β-D-xylose; BirchX, Birchwood xylan; WheatX, low viscosity wheat arabinoxylan; Man, mannan; GMan, galactomannan; pMan, para-nitrophenyl-β-D-mannose; Pect, pectin; Arab, arabinan; AraG, arabinogalactan; pAra, para-nitrophenyl-a-L-arabinose; pGal, para-nitrophenyl-β-D-galactose. The figure was edited using Multiexperiment Viewer [18]. B: Contribution of L. arvalis secretomes to the saccharification of steam-exploded WS. Biomass hydrolysis of was performed with fungal secretomes in combination with the T. reesei CL847 cocktail. The glucose released was quantified at the saccharification plateau as 30 μg of CL847 representing 100% sugar-releasing activity [19]. Values are means of six independent measures.