Figure 6

Time-course abundance of cellulose-acting enzymes in secretomes during growth on Avicel™. Abundance of cellulose-acting enzymes was determined based on the number of peptides unambiguously identified by LC-MS/MS, and the activities of endoglucanase (EG), cellobiohydrolase (CBH) and cellobiose dehydrogenase (CDH) were assayed using 2-chloro-4-nitrophenyl-β-D-cellotrioside (pCel3), para-nitrophenyl-β-D-lactose (pLac) and 2,6-dichloro-phenol-indophenol (DCPIP), respectively. For each putative enzyme, the CAZy module (sub)family is indicated. The contigs listed represent all detected secretomes members with putative EG, CBH, CDH and LPMOs activities. Peptides abundance is represented by a color scale with different strengths of purple, blue, green and red for EG, CBH, CDH and LPMOs, respectively. Activities are represented by bars (purple, blue, green and red for EG, CBH, CDH activities, respectively) and values indicated are expressed in U per mg of total proteins and are representative of triplicate measures. Error bars indicate standard errors of the mean. nd, not determined (as there is still no quantitative activity assay available for LPMOs).