Figure 2

Comparison of in silico and experimentally measured¹³C-MFA flux ranges for C. acetobutylicum [[25]. A) under wild-type biomass constraint (0.32 mmol gDW⁻¹ h⁻¹, grown in complex media) [37]. B) under reduced biomass constraint (0.07 mmol gDW⁻¹ h⁻¹ , grown in defined media) given that the data were also collected during the transition to the solventogenic phase [25]. Hexokinase (HK) and pyruvate carboxylase (PC) had their experimental values outside the model predicted ranges under the reduced biomass constraint. FVA was performed with a glucose uptake rate of 10 mmol gDW⁻¹ h⁻¹. Full reaction names can be found in the list of abbreviations.