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Table 4 Average concentration of organic acids detected in dilute acid pretreated Miscanthus hydrolysate and relative standard deviation (RSD) of intra-day and inter-day repeatability

From: Analytical method for the determination of organic acids in dilute acid pretreated biomass hydrolysate by liquid chromatography-time-of-flight mass spectrometry

 

Intra-day

Inter-day

Compound

mean (μg/ mL)

RSD (%)

mean (μg/ mL)

RSD (%)

Oxalic acid

48.4

2.0

47.8

4.3

cis-Aconitic acid

7.2

2.0

7.4

3.4

Maleic acid

11.4

3.1

11.3

1.2

Glucuronic acid

251.9

0.4

259.5

5.4

Citric acid

90.5

1.1

88.6

2.6

Galacturonic acid

607.5

0.7

581.8

3.9

Gluconic acid

218.0

2.7

215.5

1.6

Pyruvic acid

159.5

4.3

158.2

7.9

Tricarballylic acid

64.5

1.4

63.3

7.3

Glyoxylic acid

602.2

9.2

627.8

7.6

Malic acid

92.1

0.8

81.7

11.5

Malonic acid

27.8

1.5

26.7

14.9

trans-Aconitic acid*

98.4

1.7

103.3

6.6

Methylmalonic acid

7.6

4.4

7.6

18.7

Succinic acid

19.0

5.8

19.8

4.8

Glycolic acid

56.0

3.3

57.8

4.0

Lactic acid

31.1

4.9

38.0

7.2

Itaconic acid

5.7

5.8

6.5

13.4

Glutaric acid*

93.8

1.8

90.1

3.9

Fumaric acid*

105.8

2.5

103.6

2.2

2-Hydroxy-2-methylbutyric acid*

101.4

3.2

107.4

5.7

Adipic acid*

88.6

2.7

84.1

6.1

Levulinic acid

1114.3

1.0

1071.2

4.4

2-Furoic acid

12.2

8.8

13.1

12.9

  1. Hydrolysate was analyzed at day 1 (intra-day, n =3) and also at three different days (inter-day, n =3). Results respresent the concentration of the non-diluted hydrolysate (“as is”). Organic acids marked with an asterisk (*) were below the limit of quantification in the hydrolysate used. For these acids, the 1:10 diluted hydrolysate was spiked with approximately 10 μg/mL standard solution.