Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing

Table 4 Co-culturing of Y. lipolytica transformants expressing heterologous cellulases in 150 mL mineral medium containing 4 g Avicel (equivalent to 2.7% w/v) as sole carbon source

Culture of transformants	DW of Avicel-cell pellet	Avicell consumed%^[1]	DW of cells^[2]	Cell mass yield	Total FAME^[3]
Y. lipolytica[enzyme]	g		g	g DW of cells per g Avicel consumed	mg in whole pellet
[empty vector] control	4.02	0	0.02	0	N/A
[chimeric CBHI]	3.63	12.0	0.11	0.23	16.3
[chimeric CBHI] + [EGII]	3.41	20.7	0.24	0.29	24.1
[chimeric CBHI] + [EGII] + [CBHII]	3.36	23.5	0.30	0.32	28.2

Notes:^[1]Avicel degradation% was calculated as (Initial Avicel amount minus Avicel residue amount in Avicel-cell pellet) / Initial Avicel amount. The initial Avicel amount was 4 g, and the Avicel residue amount in the Avicel-cell pellet was estimated by the enzymatic hydrolysis method described in Methods section.
^[2]Dry weight (DW) of cells was calculated as DW of Avicel-cell pellets minus Avicel residue amount in Avicel-cell pellet, in which the Avicel residue amount in the Avicel-cell pellet was measured above.
^[3]The total FAME (fatty acid methyl esters) in the culture of Y. lipolytica[empty vector] could not be reliably measured due to the low amount of cell mass in the Avicel-cell pellet. N/A, not available.
Data presented are the average of duplicate samples of 120-h cultures as described in Methods section.

ISSN: 2731-3654