Synergistic hydrolysis of PCS by the purified enzyme mixture. Hydrolysis was carried out at pH 5, 45°C, with 3% (w/v) pretreated corn stover (PCS) and a protein loading of 2.55 Mg/g glucan, consisting of 40 μg of the mixed cellulolytic enzymes and 5 μg of Bgl3A in a final volume of 1 ML. The proportions (%) of the mixed cellulolytic enzymes are noted under the columns. The percent hydrolysis of PCS glucan was calculated from the amount of glucose in the hydrolysate after 48 hours. 5A, Cel5A; 6A, Cel6A; 7A, Cel7A; 7B, Cel7B; 10A, Xyl10A.