The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading

Table 1 Initial and final substrate concentrations and rate data for all fermentations shown in Figure 1

Initial [substrate] (g gluc eq/L)	Final [substrate] (g gluc eq/L)	Utilized (g gluc eq/L) (% utilization)	Maximum substrate utilization rate (g gluc eq/L/hr)
4.8	0.04	4.76 (99)	0.56
50.6	0	50.6 (100)	1.82
72.7	0.72	71.08 (99)	2.29
100.2	19.2	81 (81)	2.35
100.1	22.6	77.5 (77)	2.09
101.8	19.1	82.7 (81)	2.09
100.3	6.9	93.4 (93)	2.70
103.4	6.7	96.7 (94)	2.88

Actual concentrations of substrate are shown in gram glucose equivalents/L (g gluc eq/L) since Avicel PH105 contains xylose and insoluble residual content, and has a moisture content of around 5%. The three categories of maximum substrate utilization rates shown in Figure 1 are based on percentage utilization as shown here (approximately 80% utilization, 93% utilization and 100% utilization).

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