Simultaneous saccharification and fermentation of steam-exploded corn stover at high glucan loading and high temperature

Table 2 Experiment design for the effects of inoculation OD, nutrients, initial pH, pre-hydrolysis time, and temperature on the SSF performance of washed SECS and whole slurry by Saccharomyces cerevisiae

Parameter	Effect of OD	Effect of nutrients	Effect of initial pH	Effect of pre-hydrolysis time	Effect of temperature
Initial OD₆₀₀	1.0, 4.0, 8.0	4.0	4.0	4.0	4.0
Nutrients	50%	0%, 50%, 100%	50%	50%	50%
Initial pH	4.8	4.8	4.0, 4.8, 5.5	4.8	4.8
Pre-hydrolysis time (hours)	36	36	36	24, 36, 48	36
Temperature (°C)	36	36	36	36	30, 33, 36, 39, 42

100% nutrients was defined as 10 g/L yeast extract and 20 g/L peptone; 50% nutrients was defined as 5 g/L yeast extract and 10 g/L peptone; 0% nutrients was defined as 0 g/L yeast extract and 0 g/L peptone. OD, optical density; SSF, simultaneous saccharification and fermentation; SECS, steam-exploded corn stover.

ISSN: 2731-3654