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Table 2 Experiment design for the effects of inoculation OD, nutrients, initial pH, pre-hydrolysis time, and temperature on the SSF performance of washed SECS and whole slurry by Saccharomyces cerevisiae

From: Simultaneous saccharification and fermentation of steam-exploded corn stover at high glucan loading and high temperature

Parameter Effect of OD Effect of nutrients Effect of initial pH Effect of pre-hydrolysis time Effect of temperature
Initial OD600 1.0, 4.0, 8.0 4.0 4.0 4.0 4.0
Nutrients 50% 0%, 50%, 100% 50% 50% 50%
Initial pH 4.8 4.8 4.0, 4.8, 5.5 4.8 4.8
Pre-hydrolysis time (hours) 36 36 36 24, 36, 48 36
Temperature (°C) 36 36 36 36 30, 33, 36, 39, 42
  1. 100% nutrients was defined as 10 g/L yeast extract and 20 g/L peptone; 50% nutrients was defined as 5 g/L yeast extract and 10 g/L peptone; 0% nutrients was defined as 0 g/L yeast extract and 0 g/L peptone. OD, optical density; SSF, simultaneous saccharification and fermentation; SECS, steam-exploded corn stover.