Figure 1

Engineering E. coli for TAG biosynthesis. Heterologous proteins expressed in E. coli are highlighted in color, and the knockout of the E. coli DgkA is labeled with a red cross. Sco0958, Lppβ, and ACC correspond to diacylglycerol acyltransferase, phosphatidic acid phosphatase, and acetyl-CoA carboxylase enzymes from S. coelicolor, respectively. (1) Glycolytic pathway; (2) acetyl-CoA carboxylase; (3) malonyl-CoA:ACP transacylase; (4) glycerol-3-P dehydrogenase; (5) glycerol-3-P and lysophosphatidic acid acyltransferases; DHAP, dihydroxyacetone phosphate; FAS, fatty acid synthase; TCA, tricarboxylic acid; MDO, membrane-derived oligosaccharide.