Kinetic transcriptome analysis reveals an essentially intact induction system in a cellulase hyper-producer Trichoderma reesei strain

Poggi-Parodi, Dante; Bidard, Frédérique; Pirayre, Aurélie; Portnoy, Thomas; Blugeon, Corinne; Seiboth, Bernhard; Kubicek, Christian P; Le Crom, Stéphane; Margeot, Antoine

doi:10.1186/s13068-014-0173-z

Biotechnology for Biofuels and Bioproducts

Table 3 Inventory of the mutations affecting genes regulated during induction by lactose

From: Kinetic transcriptome analysis reveals an essentially intact induction system in a cellulase hyper-producer Trichoderma reesei strain

Transcript ID	Cluster	Annotation	Strain	Mutation	Position	Reference
NG 14 and RUT C30 strains
1751	1	Putative FAD monooxygenase	RUT C30	SNV	Promoter	[21]
54511	5	Putative POZ domain protein	RUT C30	SNV	Promoter	[21]
56077	5	Putative transcription factor	RUT C30	SNV	Promoter	[21]
77513	5	Putative fungal C6 transcription factor	RUT C30	SNV	Exon	[21]
121087	Not clustered	Putative 4Fe-4S ferredoxin domain protein	NG 14 & RUT C30	SNV	Promoter	[21]
Other cellulase improved strains
54736	1	Putative ATP-dependent RNA helicase DED1	KDG-12 PC-3-7	SNV	Promoter	[23]
58427	1	Putative ATP-dependent RNA helicase DBP2	PC-3-7	SNV	Exon	[24]
71380	1	Putative 3-hydroxy-3-methylglutaryl-coenzyme A reductase	PC-3-7	SNV	Exon	[24]
78836	1	Putative mitochondrial phosphate carrier	PC-3-7	SNV	Exon	[24]
82512	1	Putative 26S proteasome transcription factor RPN4	PC-3-7	SNV	Promoter	[24]
122036	1	Putative 40S ribosomal protein S2 RPS2	QM9414 KDG-12 PC-3-7	InDel & SNV	Promoter & Terminator	[25]
63882	4	Putative mitochondrial 3-hydroxyisobutyryl-CoA hydrolase	PC-3-7	SNV	Terminator	[24]
124031	4	Putative 20S proteasome alpha 3 subunit PRE9	PC-3-7	SNV	Promoter	[24]
6108	5	Putative SAM binding domain protein	QM9414 KDG-12 PC-3-7	InDel	Intron	Unpublished data
112390	5	Putative WD40 repeat domain protein	QM9414 KDG-12 PC-3-7	InDel	Terminator	Unpublished data
120688	5	Putative protein of unknown function	QM9414 KDG-12 PC-3-7	InDel	Exon	Unpublished data
110853	6	Putative glutathione S-transferase	PC-3-7	SNV	Terminator	[24]
77481	8	Putative D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase	PC-3-7	SNV	Exon	[24]
120749	8	Glycoside hydrolase family 1 BGL2	KDG-12 PC-3-7	SNV	Exon	[23]

We differentiate common mutations to RUT C30 and NG 14 strains from the mutations specific for RUT C30 strain. Mutations in regulated genes described in other cellulase improved strains are shown. Mutations are described as single nucleotide variation (SNV) and deletions or insertions (InDel). Affected gene region is also provided.

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ISSN: 2731-3654

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