Percentage ethanol yield at various vessel volumes. Ethanol yields based on theoretical maximum production. Handbill-style biomass weighing over 200 mL, 10 mL and 1 mL vessel volumes. Samples FP: Filter paper (Whatman number one), OCP: Office Copier Paper, YM: Yeast and Mould Media. Sample replicates n = 3. OCP and FP were included at 2.5% (w/v) dry matter along with cellulase at 20 FPU/g; YM contained glucose at 0.9% w/v. Samples were incubated for 72 hours at 25°C.