Positional preferences of acetyl esterases from different CE families towards acetylated 4-O-methyl glucuronic acid-substituted xylo-oligosaccharides

Table 3 Positional preferences of acetyl xylan esterases

Enzyme	-Xyl3- O Ac2-MeGlcA-	-Xyl2- O Ac-	-Xyl3- O Ac-	-Xyl2,3- O Ac-
TeCE1	0	12	14	74
CtCE2	0	n.d.	n.d.	0
CtCE3	0	n.d.	n.d.	0
CtCE4	0	30	70	0
AnCE5	0	24	19	57
TrCE5	0	35	33	32
OsCE6	0	26	28	46
AnCE16	n.d.	n.d.	n.d.	n.d.

Positional preference on acetylated xylo-oligosaccharides, calculated as the positional rate per relative amount of acetyl groups present (2-O, 3-O, 2,3-di-O acetylated Xylp and 3-O acetylated Xylp 2-O substituted with MeGlcA) at the start of hydrolysis. The data were normalized.
0, no activity; n.d., not determined.

ISSN: 2731-3654