Figure 2

Investigation of the efficiency and stringency of the ARAi system in C . cellulolyticum H10. (A) Determination of inducible expression of GusA in H10::pARA-GusA with X-gluc as a substrate. The blue colors of the reaction solutions indicate the induced expression of GusA. The numbered tubes contained: 1, negative control without crude enzyme sample; 2, H10::pARA-PpFbFPm; 3, H10::pARA-GusA without L-arabinose; 4 to 6, H10::pARA-GusA with 0.1, 1 and 10 g/L L-arabinose, respectively. (B) Determination of GusA activity during a time course (0 to 6 h) using MUG as a substrate. Then 1 g/L L-arabinose was used as the inducer. (C) Determination of the influence of inducer dosage (0 to 10 g/L) on GusA activity using MUG as a substrate. The induction time was 2 h.