A time course for the release of total reducing sugars from the
biomass (50 mg d.w./mL) in 50 mM citrate buffer, pH 4.8, at 50°
C. The biomass was hydrolyzed using the enzyme preparations from T. reesei (filled circles), A. cellulolyticus (open triangles), A. awamori (inverted filled triangles), the T. reesei-A. awamori blend (open circles), the A. cellulolyticus-A. awamori blend (open squares), and partially purified β-glucosidase (diamonds). Biomass suspended in buffer without enzyme preparation was used as a control (filled squares). The data were fitted into an exponential function as described in the ‘Materials and methods’ section. Standard deviation was less than 10% of the mean value, and bars were omitted for clarity.