Large-scale robot-assisted genome shuffling yields industrial Saccharomyces cerevisiae yeasts with increased ethanol tolerance

Table 1 Hybrids with improved fermentation performance identified in this study

			Ethanol production		Estimated ethanol productivity d = 7			Yield
Code	Genotype	Generation	Relative	(% ( v / v ))	(g/l)	Relative	(g/l/h)	(g/g)
H1	47	F1	107.3 ± 1.16	20.0 ± 0.22	158.1 ± 1.70	105.7 ± 1.27	0.94 ± 0.01	0.49 ± 0.00
H2	Unknown	F1	107.3 ± 0.21	20.0 ± 0.04	158.2 ± 0.32	106.7 ± 0.19	0.95 ± 0.00	0.49 ± 0.00
H3	Unknown	F3	106.2 ± 1.16	19.8 ± 0.22	156.6 ± 1.71	103.6 ± 1.32	0.93 ± 0.01	0.49 ± 0.00
H4	Unknown	F1	105.4 ± 2.35	19.4 ± 0.44	153.2 ± 0.66	104.8 ± 1.89	0.94 ± 0.02	0.49 ± 0.00
H5	12364578	F3	105.0 ± 0.41	19.6 ± 0.08	154.8 ± 0.60	104.5 ± 0.31	0.93 ± 0.00	0.48 ± 0.00
H6	Unknown	F1	104.9 ± 1.01	19.6 ± 0.19	154.6 ± 1.49	104.7 ± 0.51	0.94 ± 0.00	0.49 ± 0.01
H7	Unknown	F1	103.9 ± 0.45	19.4 ± 0.08	153.2 ± 0.66	102.3 ± 0.45	0.91 ± 0.00	0.50 ± 0.01
H8	Unknown	F3	102.0 ± 0.70	19.0 ± 0.13	150.3 ± 1.03	98.3 ± 0.59	0.88 ± 0.01	0.49 ± 0.00
H9	78	F1	100.0 ± 1.05	19.1 ± 0.20	151.0 ± 1.58	NA	NA	0.48 ± 0.01
P7	EtOH Red	-	100 ± 0.83	19.0 ± 0.40	149.7 ± 2.94	100 ± 0.84	0.90 ± 0.03	0.49 ± 0.01

Genotype indicates the predicted genotype; for hybrids generated by random mating the genotypes are unknown, whereas for targeted hybrids the parental strains are indicated. Hybrids H1 up to H7 show both increased maximal ethanol accumulation and estimated ethanol productivity relative to Ethanol Red. Data for Ethanol Red is based on 30 biological replicates. See Additional file 4 for the complete dataset.

ISSN: 2731-3654