Figure 8
Characterization of irx8 mutants complemented by CaMV35S driven GAUT12 , PdGAUT12.1 , and PdGAUT12.2 . (A) Pictures of 7-week-old wild-type (WT), and irx8 + GAUT12, irx8 + PdGAUT12.1, and irx8 + PdGAUT12.2 complemented lines. The complemented lines were classified into groups according to the phenotypes and labeled by percentage. Bar = 5 cm. (B) Images of 5-week-old rosette leaves of WT, irx8 + GAUT12, irx8 + PdGAUT12.1, irx8 + PdGAUT12.2, and irx8. Bar = 2 cm. (C) Hand-cut transverse sections of the basal stems stained with Toluidine blue O, arrows indicate collapsed xylem vessels. Bar = 50 μm. Measurements of (D) 5-week-old rosette leaf diameter and (E) 7-week-old stem height. Value = mean ± standard error (n ≥ 3), Value = mean ± standard deviation (n = 3), ***P < 0.001, significant increase of rosette leaf size or stem height compared to the irx8 mutant determined by one-way ANOVA and post hoc Bonferroni corrected t-test.