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Table 1 Total lignin content and S/G ratio of controls and PdGAUT12.1 -KD lines

From: Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock

Plant types

Lignin content (%)

S/G ratio

WT

26.13 ± 0.15

1.90 ± 0.02

V Control-1

25.82 ± 0.17

1.68 ± 0.01

V Control-2

26.68 ± 0.35

1.70 ± 0.03

V Control-3

26.00 ± 0.34

1.62 ± 0.02

V Control-4

26.45 ± 0.21

1.73 ± 0.03

V Control-5

26.43 ± 0.42

1.85 ± 0.03

V Control-6

26.24 ± 0.16

1.85 ± 0.04

V Control-7

25.61 ± 0.40

1.62 ± 0.07

V Control-8

26.30 ± 0.25

1.78 ± 0.05

AB30.1

25.53 ± 0.50

2.00 ± 0.03*

AB30.2

25.99 ± 0.39

1.96 ± 0.02*

AB30.3

26.37 ± 0.15

1.98 ± 0.02 *

AB30.4

26.61 ± 0.26

1.98 ± 0.03*

AB30.5

26.84 ± 0.21

1.97 ± 0.03*

AB30.6

26.11 ± 0.41

2.02 ± 0.04*

AB30.7

26.43 ± 0.38

1.97 ± 0.02*

AB30.8

25.78 ± 0.37

1.97 ± 0.02*

AB30.9

26.44 ± 0.63

2.04 ± 0.02*

AB30.10

26.38 ± 0.24

1.98 ± 0.02*

AB30.11

26.28 ± 0.17

2.03 ± 0.02*

  1. Lignin content is expressed as percentage (wt/wt). S/G ratios were determined by summing the intensity of the syringyl peaks at 154, 167, 168, 182, 194, 208, and 210 and dividing by the sum of intensity of guaiacyl peaks 124, 137, 138, 150, 164, and 178 (see methods for details, Additional file 9). Mean ± SE, Significant P values are expressed as *P < 0.05, **P < 0.001 as calculated by statistical analysis using Statistica 5.0 with one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. n = 25 for wild-type P. deltoides, n = 10 to 15 for vector control (v control-1 to v control-8) and PdGAUT12.1-KD lines (AB30.1-AB30.11).
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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