Dynamic metabolic profiling of the marine microalga Chlamydomonas sp. JSC4 and enhancing its oil production by optimizing light intensity

Table 6 Biodiesel properties of Chlamydomonas sp. JSC4

Biodiesel property	150 μmol m ⁻² s ⁻¹	300 μmol m ⁻² s ⁻¹	500 μmol m ⁻² s ⁻¹	US standard (ASTM D6751-08)	European standard (EN 14214)
Kinematic viscosity^a	4.71	4.72	4.74	1.9 to 6.0	3.5 to 5.0
Specific gravity^b	0.88	0.88	0.88	0.85 to 0.90	0.86 to 0.90
Cetane number^c	57.60	57.69	57.99	min47	min51
Iodine number^d	71.54	70.53	67.24	-	max120
Average unsaturation	0.79	0.78	0.73	-	-

The fatty acids produced from dried biomass of Chlamydomonas sp. JSC4 cultured for 5 days under conditions of nitrogen depletion and different light intensities. The relationship between average degree of unsaturation of fatty acids and biodiesel properties as reported by Song et al. [32] are shown. Values are the mean of three replicated experiments (CO₂ aeration = 2.0%; CO₂ flow rate = 0.1 vvm.).
^aCalculated from the following equation: Kinematic viscosity (mm² s⁻¹, at 40°C) = ⁻0.6316 × average unsaturation + 5.2065.
^bCalculated from the following equation: Specific gravity (kg L⁻¹) = 0.0055 × average unsaturation + 0.8726.
^cCalculated from the following equation: Cetane number = ⁻6.6684 × average unsaturation + 62.876.
^dCalculated from the following equation: Iodine number (gl₂ g⁻¹) = 74.373 × average unsaturation + 12.71.

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