Total propane produced by the engineered
. Total propane accumulated over 4 h of reaction under assay conditions performed in GC vials for the pathway-engineered E. coli cells, after overexpressing with ADO. The experimental protocol is described in the ‘Materials and methods’ section. Error bars are standard deviation (n = 4). ADO, aldehyde deformylating oxygenase; IPTG, isopropyl β-D-1-thiogalactopyranoside.