Figure 4

Reduced sensitivity to acetic acid in optimal defined medium. Sensitivity of S. stipitis NRRL Y-7124 in ODM to increasing acetic acid concentrations at 2 (A), 6 (B, and 10 g/L (C) is compared to relative tolerance of adapted colony 5 at the same acetic concentrations ((D, (E) and (F), respectively). The following time course data are shown: biomass (red squares), glucose (black circles and dashed line), xylose (blue circles and solid line), xylitol (purple diamonds), acetic acid (red stars), ethanol (green triangles), viable cells (inverted aqua triangles).