Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

Slininger, Patricia J; Shea-Andersh, Maureen A; Thompson, Stephanie R; Dien, Bruce S; Kurtzman, Cletus P; Balan, Venkatesh; da Costa Sousa, Leonardo; Uppugundla, Nirmal; Dale, Bruce E; Cotta, Michael A

doi:10.1186/s13068-015-0239-6

Biotechnology for Biofuels and Bioproducts

Table 3 Summary of relative performance indexes (RPI _overall ) of isolates

From: Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

		SGH-N1 and SGH-N2				SGH-N1, SGH-N2, and AFEX CSH
Screen number	Isolate designation	RPI _overall	s	Rel. s (%)	Statistic group	RPI _overall	s	Rel. s (%)	Statistic group
1	Y-7124	38.3	23.6	61.5	D	40.0	23.0	57.3	C
2	Y-7124 S80E40-2	48.6	24.7	50.8	C	47.0	22.2	47.1	C
3	Y-7124 S90E40-1	61.8	15.6	25.2	A	61.1	14.7	24.0	A
4	Colony 5 S90E40-5	40.1	12.6	31.5	C
5	Colony 5 S100E40-5	53.6	11.8	22.0	B	51.1	12.3	24.0	B
6	2A.1.53R S90E40-4	54.0	19.0	35.2	B	48.8	12.4	25.5	C
7	2A.1.53R S100E40-5	52.3	16.6	31.7	B
8	Y-7124 GP-1	50.7	23.9	47.1	B	46.2	23.1	50.1	C
9	Y-7124 GP-5	59.8	15.7	26.3	B	55.3	17.6	31.9	B
10	Colony 5 GP-2	58.9	10.1	17.2	B	51.9	17.4	33.5	B
11	Colony 5 GP-6	59.9	15.4	25.8	B	54.8	17.7	32.3	B
12	2A.1.53R S90E40-2	34.2	17.1	50.1	D
13	2A.1.53R S100E40-1	52.2	10.5	20.1	B	51.5	9.5	18.4	B
14	Y-7124-6	58.2	16.2	27.9	B	62.1	17.0	27.3	A
15	Y-7124-10	57.6	6.6	11.4	B	55.9	6.7	11.9	B
16	2A.1.53R-1	58.3	10.2	17.4	B	54.4	14.0	25.8	B
17	2A.1.53R-6	49.4	13.1	26.5	C
18	Colony 5-3	53.3	11.7	22.0	B	46.7	17.2	36.8	C
19	Colony 5-4	41.6	16.7	40.1	C
20	Y-7124 GP3-1	32.0	18.2	56.8	D
21	Y-7124 GP3-5	54.4	18.9	34.7	B	48.1	21.2	44.1	C
22	Colony 5 25%-2 N	46.8	29.3	62.6	C
23	2A.1.53R 25%-1 N	47.8	13.8	28.9	C
24	2A.1.53R 25%-2	51.5	16.7	32.4	B
25	2A.44R-E20-C1	48.6	15.4	31.7	C	54.4	18.4	33.8	B
26	2A.44R-E40-C2	55.0	29.7	54.0	B
27	2A.1.53R-E20-C1	61.5	23.7	38.6	A	69.3	27.7	39.9	A
28	2A.1.53R-E30-C3	63.3	17.6	27.9	A	61.4	16.2	26.4	A
29	2A.30R2-E30-C5	40.5	52.9	130.6	C	45.8	48.5	105.9	C
30	2A.30R2-E40-C5	25.5	81.4	319.3	D	32.3	74.7	231.4	D
31	3A.1.57-E20-C1	32.2	13.7	42.5	D
32	3A.1.57-E30-C1	50.2	11.2	22.3	B	43.4	17.7	40.8	C
33	Colony 5	63.0	14.8	23.5	A	68.4	17.4	25.4	A

Isolates in italics are considered superior based on high overall RPI across hydrolyzate types, low relative standard deviation (Rel. s), and/or exceedingly high RPI on at least one hydrolyzate type as seen in Figure 10A.
Strains are ranked based on ethanol yield and xylose uptake rate in screens on dilute acid-pretreated switchgrass enzyme hydrolyzates with two different nutrient supplements (SGH-N1 and SGH-N2) or over all three hydrolyzates including AFEX-pretreated corn stover enzyme hydrolyzates.

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