Figure 6
P tcu1 -xyr1 displayed a full production of cellulases on non-inducing carbon sources. (A) SDS-PAGE analysis and pNPC hydrolytic activities of the extracellular proteins from QM9414 (left panel) and P tcu1 -xyr1 (right panel) strains grown on 1% (wt/vol) glucose without exogenous addition of CuSO4. (B) The same as in (A), but the strains were grown on 1% (wt/vol) glycerol. (C) Filter paper hydrolytic activity of the cellulases produced by P tcu1 -xyr1 on 1% (wt/vol) glycerol or those produced by QM9414 strain on 1% (wt/vol) Avicel or glycerol cultured with or without CuSO4. A significant difference (P < 0.05) was detected between the produced cellulases of P tcu1 -xyr1 on 1% (wt/vol) glycerol without CuSO4 and those of QM9414 on 1% (wt/vol) glycerol. (D) The extracellular pNPC hydrolytic activity of P tcu1 -xyr1 cultured on 1% (wt/vol) glycerol in the presence of the indicated concentrations of CuSO4. Equal amount of culture supernatant relative to biomass was measured for all the assays. Error bars are the SD from at least two biological replicates.