Figure 7
qRT-PCR analysis of the transcription of xyr1 and cel7a in strains P tcu1 -xyr1 and QM9414. (A) Transcript abundance of xyr1 of P tcu1 -xyr1 on non-inducing carbon sources compared with those of QM9414 on Avicel in the presence or absence of 10 μM CuSO4. (B) Levels of cel7a mRNA in P tcu1 -xyr1 on non-inducing carbon sources compared with those in QM9414 on Avicel with or without addition of 10 μM CuSO4. Significant differences (P < 0.05) were detected for the transcription of xyr1 between P tcu1 -xyr1 on glucose or glycerol without CuSO4 and QM9414 on Avicel at all three indicated time points. For the transcription of cel7a, a significant difference (P < 0.001) was detected between P tcu1 -xyr1 at 48 h on glucose and those in QM9414 on Avicel. Error bars are the SD from three biological replicates.