Fig. 1

Extractable lipid class and FAME profiles for cultures of C. vulgaris re-suspended into medium depleted of nitrogen and supplemented with various bicarbonate salts. Final concentrations of bicarbonate salts per experimental condition: 0 mM HCO₃ ⁻ (control), 50 mM ACS grade NaHCO₃ (grade 1), 50 mM industrial grade NaHCO₃ (grade 2), 50 mM KHCO₃, 50 mM Na₂CO₃, and 25 mM NaHCO₃∙Na₂CO₃ (25 mM of sesquicarbonate was used to provide equimolar carbon). Values are reported for the completion of the experiment (n = 3). All values expressed as weight percent (% weight extractable lipid or weight FAME/weight biomass)